Intracellular Products Release
The second stage of downstream processing is called Intracellular Products Release. During the
downstream processing, several biotechnological products have to be released and isolated from the cell.
The isolation and release of the biotechnological products are done with the help of Intracellular Products
Biotechnological compounds/products that can be removed from the cell through the technique of
Intracellular Products Release are:
Methods of the Intracellular Products Release
There are several methods that are used in Stage II of downstream processing. The main technique of
the Intracellular Products Release is called Cell Disruption, which has its methods.
The methods used in cell disruption are the following:
Cell Disruption Physical Methods
The ultrasonic disintegration of the biotechnological products used for DP is one of the most used
methods of the Intracellular Products Release’s physical method of Cell Disruption.
The only disadvantage of Ultrasonication is that it can’t be used every day in the laboratory because it
requires the use of expensive chemicals and expensive laboratory equipment.
The second technique of Cell Disruption method of the second stage of downstream processing –
Intracellular Release, is called Osmotic Shock.
The Osmotic Shock method is used for suspending the Gram-negative bacterias’ hydrolytic enzymes and
proteins bound to them.
Chemical Methods of the Cell Disruption
The alkalines chemical method of Cell Disruption technique is used for extracting the bacterial proteins.
For instance, with the help of the alkalines method, the growth hormone can be released from the Vitamin