Tag Archives: layer chromatography

Types of chromatography

Types of chromatography is a wide aspect on its own. This is because the types are arrived at depending on a couple of factors among them the technique employed, the physical characteristics of the mixtures/ components and others. In light of all the factors put in to consideration, we can say we have around 12 types of chromatography.

What are the types of chromatography?

Below are the 12 types of chromatography with a little explanation of each type:

  • High performance liquid chromatography- this type applies the principle of surface area to volume ratio. The mobile phase is pumped into the column at a pre-set pressure. The column particles are very small increasing the surface area to volume ratio hence better separation takes place
  • Gas chromatography- as the name suggests, this type has a particular interest in gas. This is because it uses gas for the mobile phase. The gas used has to be pure i.e. doesn’t have any impurities.
  • Ion- exchange chromatography- this type is dependent on the charge of ions in the mobile phase. Ions in the sample with the same charge will be eluted as like charges repel
  • Size exclusion chromatography- this method works around the size of particles in the component. The largest particles are eluted first and the smaller ones follow
  • Thin layer chromatography- like the name, this type has the stationary phase as a thin layer
  • Paper chromatography- here, we use a rectangular or circular paper as the column.
  • Affinity chromatography
  • Liquid chromatography combined with mass spectroscopy
  • Gas chromatography combined with mass spectroscopy
  • Ultra high performance chromatograph
  • High performance thin layer chromatography- a more efficient type of TLC
  • Column chromatography- the mobile phase is poured through a column that in turn allows separation of the sample