Tag Archives: Liquid chromatography

Chromatography and its abundant uses

Chromatography may not seem interesting for people who are away from the sciences world. This process affects daily lives in many ways. It is useful to separate a compound into various components. Chromatography plays a crucial role in the forensics, athlete testing, food regulation and also in favorite alcoholic drinks control.


Chromatography everyday uses

  • If you are into Law and Order and CSI programs, it is helpful for the investigation of criminal cases.  It helps in analysis of cloth or blood samples, blood testing, arson verification, etc. This process helps in determining alcohol, poisonous substances or drugs levels in the body.
  • Chromatography offers accuracy and precision as it can identify substances that are in the bloodstream.  Thus it is valuable for doping testing or even to check the drugs in athletes that enhance performance.
  • Chromatography can trace meat as well. The efficacy in the food is of immense use in analyzing.


Ebola Immunization


Specializing in variety of flippant matters such as the alcohol quality is one of the best benefits of chromatography as it helps saving millions of lives and is truly helpful. Ebola virus, the deadly one claimed more than 5000 lives from the last year after its outbreak and caused panic everywhere, especially in Guinea, Sierra Leone and Liberia, to places it is largely confined.
The scientists keep on making attempts to fight off the disease, but only chromatography has shown itself that it is incredibly useful in identifying the antibodies that are more effective and helps in neutralizing Ebola. There are no drugs that are validated as yet conclusively, thus it was the key factor in the development of this experimental immunization Zmapp. Nevertheless, it is expected to continue in the ongoing research to be used so that chromatography helps in determining the actual reason.

Techniques Employed In Chromatography

The separation of mixtures using chromatography is different in every type of chromatography. This is because different mixtures are made of components that are of different characteristics. Some mixtures are made of gases while others are made of liquids. For this reason, the type of chromatography will be different in different cases.

The techniques employed are however similar across all the types. This is mainly because the different types are all based on the same principles. The objective is also the same as they all seek to separate individual components.

The techniques of chromatography are as follows:-

  • Stationary phase- as the name suggests, the phase is motionless and allows the sample to move over it. It can either be in solid or liquid state. If in solid state, it is important for the particles to be uniform in size and shape. When the phase is liquid, it has to be spread uniformly across a solid background.
  • Mobile phase- this is what aids the mixture in question to move across the stationary/ motionless phase. It is either liquid or in gaseous state to ensure movement. It must always be opposite in polarity to the stationary phase. This means if the stationary phase is polar, the mobile phase should be non- polar and vice versa. It should also be pure
  • Flow rate- the rate at which the mobile phase flows over the stationary phase should always be kept constant. This ensures reliable results
  • Temperature- temperature should be constant to ensure it doesn’t alter the flow rate. High flow rate leads to faster separation but colour bands can be very close.
  • Treating of the sample- some samples need to be treated to ease separation. This is basically the chemical alteration of a sample before or after it is separated.